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Using single nuclei for RNA-seq to capture the transcriptome of postmortem neurons.
Krishnaswami SR, Grindberg RV, Novotny M, Venepally P, Lacar B, Bhutani K, Linker SB, Pham S, Erwin JA, Miller JA, Hodge R, McCarthy JK, Kelder M, McCorrison J, Aevermann BD, Fuertes FD, Scheuermann RH, Lee J, Lein ES, Schork N, McConnell MJ, Gage FH, Lasken RS. Krishnaswami SR, et al. Among authors: lasken rs. Nat Protoc. 2016 Mar;11(3):499-524. doi: 10.1038/nprot.2016.015. Epub 2016 Feb 18. Nat Protoc. 2016. PMID: 26890679 Free PMC article.
Nuclear RNA-seq of single neurons reveals molecular signatures of activation.
Lacar B, Linker SB, Jaeger BN, Krishnaswami SR, Barron JJ, Kelder MJE, Parylak SL, Paquola ACM, Venepally P, Novotny M, O'Connor C, Fitzpatrick C, Erwin JA, Hsu JY, Husband D, McConnell MJ, Lasken R, Gage FH. Lacar B, et al. Nat Commun. 2016 Apr 19;7:11022. doi: 10.1038/ncomms11022. Nat Commun. 2016. PMID: 27090946 Free PMC article.
RNA-sequencing from single nuclei.
Grindberg RV, Yee-Greenbaum JL, McConnell MJ, Novotny M, O'Shaughnessy AL, Lambert GM, Araúzo-Bravo MJ, Lee J, Fishman M, Robbins GE, Lin X, Venepally P, Badger JH, Galbraith DW, Gage FH, Lasken RS. Grindberg RV, et al. Among authors: lasken rs. Proc Natl Acad Sci U S A. 2013 Dec 3;110(49):19802-7. doi: 10.1073/pnas.1319700110. Epub 2013 Nov 18. Proc Natl Acad Sci U S A. 2013. PMID: 24248345 Free PMC article.
PRODUCTION OF A PRELIMINARY QUALITY CONTROL PIPELINE FOR SINGLE NUCLEI RNA-SEQ AND ITS APPLICATION IN THE ANALYSIS OF CELL TYPE DIVERSITY OF POST-MORTEM HUMAN BRAIN NEOCORTEX.
Aevermann B, McCorrison J, Venepally P, Hodge R, Bakken T, Miller J, Novotny M, Tran DN, Diezfuertes F, Christiansen L, Zhang F, Steemers F, Lasken RS, Lein ED, Schork N, Scheuermann RH. Aevermann B, et al. Among authors: lasken rs. Pac Symp Biocomput. 2017;22:564-575. doi: 10.1142/9789813207813_0052. Pac Symp Biocomput. 2017. PMID: 27897007 Free PMC article.
Transcriptomic evidence that von Economo neurons are regionally specialized extratelencephalic-projecting excitatory neurons.
Hodge RD, Miller JA, Novotny M, Kalmbach BE, Ting JT, Bakken TE, Aevermann BD, Barkan ER, Berkowitz-Cerasano ML, Cobbs C, Diez-Fuertes F, Ding SL, McCorrison J, Schork NJ, Shehata SI, Smith KA, Sunkin SM, Tran DN, Venepally P, Yanny AM, Steemers FJ, Phillips JW, Bernard A, Koch C, Lasken RS, Scheuermann RH, Lein ES. Hodge RD, et al. Among authors: lasken rs. Nat Commun. 2020 Mar 3;11(1):1172. doi: 10.1038/s41467-020-14952-3. Nat Commun. 2020. PMID: 32127543 Free PMC article.
Predicting the functional states of human iPSC-derived neurons with single-cell RNA-seq and electrophysiology.
Bardy C, van den Hurk M, Kakaradov B, Erwin JA, Jaeger BN, Hernandez RV, Eames T, Paucar AA, Gorris M, Marchand C, Jappelli R, Barron J, Bryant AK, Kellogg M, Lasken RS, Rutten BP, Steinbusch HW, Yeo GW, Gage FH. Bardy C, et al. Among authors: lasken rs. Mol Psychiatry. 2016 Nov;21(11):1573-1588. doi: 10.1038/mp.2016.158. Epub 2016 Oct 4. Mol Psychiatry. 2016. PMID: 27698428 Free PMC article.
Corrigendum: Nuclear RNA-seq of single neurons reveals molecular signatures of activation.
Lacar B, Linker SB, Jaeger BN, Krishnaswami SR, Barron JJ, Kelder MJ, Parylak SL, Paquola AC, Venepally P, Novotny M, O'Connor C, Fitzpatrick C, Erwin JA, Hsu JY, Husband D, McConnell MJ, Lasken R, Gage FH. Lacar B, et al. Nat Commun. 2016 Jun 14;7:12020. doi: 10.1038/ncomms12020. Nat Commun. 2016. PMID: 27295945 Free PMC article. No abstract available.
L1-associated genomic regions are deleted in somatic cells of the healthy human brain.
Erwin JA, Paquola AC, Singer T, Gallina I, Novotny M, Quayle C, Bedrosian TA, Alves FI, Butcher CR, Herdy JR, Sarkar A, Lasken RS, Muotri AR, Gage FH. Erwin JA, et al. Among authors: lasken rs. Nat Neurosci. 2016 Dec;19(12):1583-1591. doi: 10.1038/nn.4388. Epub 2016 Sep 12. Nat Neurosci. 2016. PMID: 27618310 Free PMC article.
Cell type discovery and representation in the era of high-content single cell phenotyping.
Bakken T, Cowell L, Aevermann BD, Novotny M, Hodge R, Miller JA, Lee A, Chang I, McCorrison J, Pulendran B, Qian Y, Schork NJ, Lasken RS, Lein ES, Scheuermann RH. Bakken T, et al. Among authors: lasken rs. BMC Bioinformatics. 2017 Dec 21;18(Suppl 17):559. doi: 10.1186/s12859-017-1977-1. BMC Bioinformatics. 2017. PMID: 29322913 Free PMC article.
73 results