High-level production, chemical modification and site-directed mutagenesis of a cephalosporin C acylase from Pseudomonas strain N176.
Ishii Y, Saito Y, Fujimura T, Sasaki H, Noguchi Y, Yamada H, Niwa M, Shimomura K.
Ishii Y, et al. Among authors: niwa m.
Eur J Biochem. 1995 Jun 1;230(2):773-8.
Eur J Biochem. 1995.
PMID: 7607251
Free article.
Tyr270 in the acylase is reported to play an important role in the interaction with glutarylcephalosporanic acid, as determined from the reaction with an affinity-label reagent, 7 beta-(6-bromohexanoylamido) cephalosporanic acid [Ishii, Y., Saito, Y., Sasaki, H., Uchiyama, F., Ha …
Tyr270 in the acylase is reported to play an important role in the interaction with glutarylcephalosporanic acid, as determined from the rea …