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When monoclonal antibodies are not monospecific: Hybridomas frequently express additional functional variable regions.
Bradbury ARM, Trinklein ND, Thie H, Wilkinson IC, Tandon AK, Anderson S, Bladen CL, Jones B, Aldred SF, Bestagno M, Burrone O, Maynard J, Ferrara F, Trimmer JS, Görnemann J, Glanville J, Wolf P, Frenzel A, Wong J, Koh XY, Eng HY, Lane D, Lefranc MP, Clark M, Dübel S. Bradbury ARM, et al. Among authors: wilkinson ic. MAbs. 2018 May/Jun;10(4):539-546. doi: 10.1080/19420862.2018.1445456. Epub 2018 Mar 29. MAbs. 2018. PMID: 29485921 Free PMC article.
Conformational heterogeneity in antibody-protein antigen recognition: implications for high affinity protein complex formation.
Addis PW, Hall CJ, Bruton S, Veverka V, Wilkinson IC, Muskett FW, Renshaw PS, Prosser CE, Carrington B, Lawson ADG, Griffin R, Taylor RJ, Waters LC, Henry AJ, Carr MD. Addis PW, et al. Among authors: wilkinson ic. J Biol Chem. 2014 Mar 7;289(10):7200-7210. doi: 10.1074/jbc.M113.492215. Epub 2014 Jan 16. J Biol Chem. 2014. PMID: 24436329 Free PMC article.