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FRET-enhanced photostability allows improved single-molecule tracking of proteins and protein complexes in live mammalian cells.
Basu S, Needham LM, Lando D, Taylor EJR, Wohlfahrt KJ, Shah D, Boucher W, Tan YL, Bates LE, Tkachenko O, Cramard J, Lagerholm BC, Eggeling C, Hendrich B, Klenerman D, Lee SF, Laue ED. Basu S, et al. Among authors: needham lm. Nat Commun. 2018 Jun 28;9(1):2520. doi: 10.1038/s41467-018-04486-0. Nat Commun. 2018. PMID: 29955052 Free PMC article.
Single-molecule visualization of DNA G-quadruplex formation in live cells.
Di Antonio M, Ponjavic A, Radzevičius A, Ranasinghe RT, Catalano M, Zhang X, Shen J, Needham LM, Lee SF, Klenerman D, Balasubramanian S. Di Antonio M, et al. Among authors: needham lm. Nat Chem. 2020 Sep;12(9):832-837. doi: 10.1038/s41557-020-0506-4. Epub 2020 Jul 20. Nat Chem. 2020. PMID: 32690897 Free PMC article.
vLUME: 3D virtual reality for single-molecule localization microscopy.
Spark A, Kitching A, Esteban-Ferrer D, Handa A, Carr AR, Needham LM, Ponjavic A, Santos AM, McColl J, Leterrier C, Davis SJ, Henriques R, Lee SF. Spark A, et al. Among authors: needham lm. Nat Methods. 2020 Nov;17(11):1097-1099. doi: 10.1038/s41592-020-0962-1. Epub 2020 Oct 12. Nat Methods. 2020. PMID: 33046895 Free PMC article.
ThX - a next-generation probe for the early detection of amyloid aggregates.
Needham LM, Weber J, Varela JA, Fyfe JWB, Do DT, Xu CK, Tutton L, Cliffe R, Keenlyside B, Klenerman D, Dobson CM, Hunter CA, Müller KH, O'Holleran K, Bohndiek SE, Snaddon TN, Lee SF. Needham LM, et al. Chem Sci. 2020 Feb 21;11(18):4578-4583. doi: 10.1039/c9sc04730a. Chem Sci. 2020. PMID: 34122915 Free PMC article.
Single-molecule fluorescence detection of a tricyclic nucleoside analogue.
Samaan GN, Wyllie MK, Cizmic JM, Needham LM, Nobis D, Ngo K, Andersen S, Magennis SW, Lee SF, Purse BW. Samaan GN, et al. Among authors: needham lm. Chem Sci. 2020 Dec 28;12(7):2623-2628. doi: 10.1039/d0sc03903a. Chem Sci. 2020. PMID: 34164030 Free PMC article.
POLCAM: instant molecular orientation microscopy for the life sciences.
Bruggeman E, Zhang O, Needham LM, Körbel M, Daly S, Cheetham M, Peters R, Wu T, Klymchenko AS, Davis SJ, Paluch EK, Klenerman D, Lew MD, O'Holleran K, Lee SF. Bruggeman E, et al. Among authors: needham lm. Nat Methods. 2024 Oct;21(10):1873-1883. doi: 10.1038/s41592-024-02382-8. Epub 2024 Oct 7. Nat Methods. 2024. PMID: 39375574 Free PMC article.
23 results