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Antibody-free, targeted mass-spectrometric approach for quantification of proteins at low picogram per milliliter levels in human plasma/serum.
Shi T, Fillmore TL, Sun X, Zhao R, Schepmoes AA, Hossain M, Xie F, Wu S, Kim JS, Jones N, Moore RJ, Pasa-Tolić L, Kagan J, Rodland KD, Liu T, Tang K, Camp DG 2nd, Smith RD, Qian WJ. Shi T, et al. Among authors: zhao r. Proc Natl Acad Sci U S A. 2012 Sep 18;109(38):15395-400. doi: 10.1073/pnas.1204366109. Epub 2012 Sep 4. Proc Natl Acad Sci U S A. 2012. PMID: 22949669 Free PMC article.
Long-gradient separations coupled with selected reaction monitoring for highly sensitive, large scale targeted protein quantification in a single analysis.
Shi T, Fillmore TL, Gao Y, Zhao R, He J, Schepmoes AA, Nicora CD, Wu C, Chambers JL, Moore RJ, Kagan J, Srivastava S, Liu AY, Rodland KD, Liu T, Camp DG 2nd, Smith RD, Qian WJ. Shi T, et al. Among authors: zhao r. Anal Chem. 2013 Oct 1;85(19):9196-203. doi: 10.1021/ac402105s. Epub 2013 Sep 19. Anal Chem. 2013. PMID: 24004026 Free PMC article.
Sensitive targeted quantification of ERK phosphorylation dynamics and stoichiometry in human cells without affinity enrichment.
Shi T, Gao Y, Gaffrey MJ, Nicora CD, Fillmore TL, Chrisler WB, Gritsenko MA, Wu C, He J, Bloodsworth KJ, Zhao R, Camp DG 2nd, Liu T, Rodland KD, Smith RD, Wiley HS, Qian WJ. Shi T, et al. Among authors: zhao r. Anal Chem. 2015 Jan 20;87(2):1103-10. doi: 10.1021/ac503797x. Epub 2014 Dec 31. Anal Chem. 2015. PMID: 25517423 Free PMC article.
Automated Nanoflow Two-Dimensional Reversed-Phase Liquid Chromatography System Enables In-Depth Proteome and Phosphoproteome Profiling of Nanoscale Samples.
Dou M, Tsai CF, Piehowski PD, Wang Y, Fillmore TL, Zhao R, Moore RJ, Zhang P, Qian WJ, Smith RD, Liu T, Kelly RT, Shi T, Zhu Y. Dou M, et al. Among authors: zhao r. Anal Chem. 2019 Aug 6;91(15):9707-9715. doi: 10.1021/acs.analchem.9b01248. Epub 2019 Jul 9. Anal Chem. 2019. PMID: 31241912 Free PMC article.
High-Throughput Single Cell Proteomics Enabled by Multiplex Isobaric Labeling in a Nanodroplet Sample Preparation Platform.
Dou M, Clair G, Tsai CF, Xu K, Chrisler WB, Sontag RL, Zhao R, Moore RJ, Liu T, Pasa-Tolic L, Smith RD, Shi T, Adkins JN, Qian WJ, Kelly RT, Ansong C, Zhu Y. Dou M, et al. Among authors: zhao r. Anal Chem. 2019 Oct 15;91(20):13119-13127. doi: 10.1021/acs.analchem.9b03349. Epub 2019 Sep 25. Anal Chem. 2019. PMID: 31509397 Free PMC article.
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