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Page 1
Gateway cloning is compatible with protein secretion from Pichia pastoris.
Esposito D, Gillette WK, Miller DA, Taylor TE, Frank PH, Hu R, Bekisz J, Hernandez J, Cregg JM, Zoon KC, Hartley JL. Esposito D, et al. Protein Expr Purif. 2005 Apr;40(2):424-8. doi: 10.1016/j.pep.2004.12.006. Protein Expr Purif. 2005. PMID: 15766886 Free PMC article.
Gateway cloning for protein expression.
Esposito D, Garvey LA, Chakiath CS. Esposito D, et al. Methods Mol Biol. 2009;498:31-54. doi: 10.1007/978-1-59745-196-3_3. Methods Mol Biol. 2009. PMID: 18988017
Purify First: rapid expression and purification of proteins from XMRV.
Gillette WK, Esposito D, Taylor TE, Hopkins RF, Bagni RK, Hartley JL. Gillette WK, et al. Among authors: esposito d. Protein Expr Purif. 2011 Apr;76(2):238-47. doi: 10.1016/j.pep.2010.12.003. Epub 2010 Dec 10. Protein Expr Purif. 2011. PMID: 21146612
Mammalian cell transient expression, non-affinity purification, and characterization of human recombinant IGFBP7, an IGF-1 targeting therapeutic protein.
Jiang MS, Yang X, Esposito D, Nelson E, Yuan J, Hopkins RF, Broadt T, Xiao Z, Colantonio S, Prieto DA, Welch AR, Creekmore SP, Mitra G, Zhu J. Jiang MS, et al. Among authors: esposito d. Int Immunopharmacol. 2015 Dec;29(2):476-487. doi: 10.1016/j.intimp.2015.10.008. Epub 2015 Oct 23. Int Immunopharmacol. 2015. PMID: 26474694
Farnesylated and methylated KRAS4b: high yield production of protein suitable for biophysical studies of prenylated protein-lipid interactions.
Gillette WK, Esposito D, Abreu Blanco M, Alexander P, Bindu L, Bittner C, Chertov O, Frank PH, Grose C, Jones JE, Meng Z, Perkins S, Van Q, Ghirlando R, Fivash M, Nissley DV, McCormick F, Holderfield M, Stephen AG. Gillette WK, et al. Among authors: esposito d. Sci Rep. 2015 Nov 2;5:15916. doi: 10.1038/srep15916. Sci Rep. 2015. PMID: 26522388 Free PMC article.
843 results