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Application of simple fed-batch technique to high-level secretory production of insulin precursor using Pichia pastoris with subsequent purification and conversion to human insulin.
Gurramkonda C, Polez S, Skoko N, Adnan A, Gäbel T, Chugh D, Swaminathan S, Khanna N, Tisminetzky S, Rinas U. Gurramkonda C, et al. Among authors: polez s. Microb Cell Fact. 2010 May 12;9:31. doi: 10.1186/1475-2859-9-31. Microb Cell Fact. 2010. PMID: 20462406 Free PMC article.