Protocol for producing hyperpolarized 13C-bicarbonate for clinical MRI of extracellular pH in aggressive tumors.
Mu C, Liu X, Riselli A, Slater J, Escobar E, Dang D, Drapeau S, Delos Santos R, Andosca S, Nguyen H, Larson PEZ, Bok R, Vigneron DB, Kurhanewicz J, Wilson DM, Flavell RR.
Mu C, et al.
STAR Protoc. 2024 Sep 20;5(3):103091. doi: 10.1016/j.xpro.2024.103091. Epub 2024 Jun 28.
STAR Protoc. 2024.
PMID: 38943645
Free PMC article.
Here, we present a protocol for preparing a hyperpolarized (HP) (13)C-bicarbonate tissue pH MRI imaging contrast agent to detect aggressive tumors. We describe the steps for the formulation and polarization of a precursor molecule (13)C-glycerol carbonate ((13)C …
Here, we present a protocol for preparing a hyperpolarized (HP) (13)C-bicarbonate tissue pH MRI imaging contrast agent to detect aggr …