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Multiplex genome editing eliminates lactate production without impacting growth rate in mammalian cells.
Hefzi H, Martínez-Monge I, Marin de Mas I, Cowie NL, Toledo AG, Noh SM, Karottki KJC, Decker M, Arnsdorf J, Camacho-Zaragoza JM, Kol S, Schoffelen S, Pristovšek N, Hansen AH, Miguez AA, Bjørn SP, Brøndum KK, Javidi EM, Jensen KL, Stangl L, Kreidl E, Kallehauge TB, Ley D, Ménard P, Petersen HM, Sukhova Z, Bauer A, Casanova E, Barron N, Malmström J, Nielsen LK, Lee GM, Kildegaard HF, Voldborg BG, Lewis NE. Hefzi H, et al. Among authors: lee gm. Nat Metab. 2025 Jan 14. doi: 10.1038/s42255-024-01193-7. Online ahead of print. Nat Metab. 2025. PMID: 39809975
Multiplex secretome engineering enhances recombinant protein production and purity.
Kol S, Ley D, Wulff T, Decker M, Arnsdorf J, Schoffelen S, Hansen AH, Jensen TL, Gutierrez JM, Chiang AWT, Masson HO, Palsson BO, Voldborg BG, Pedersen LE, Kildegaard HF, Lee GM, Lewis NE. Kol S, et al. Among authors: lee gm. Nat Commun. 2020 Apr 20;11(1):1908. doi: 10.1038/s41467-020-15866-w. Nat Commun. 2020. PMID: 32313013 Free PMC article.
Mitigating Clonal Variation in Recombinant Mammalian Cell Lines.
Lee JS, Kildegaard HF, Lewis NE, Lee GM. Lee JS, et al. Among authors: lee gm. Trends Biotechnol. 2019 Sep;37(9):931-942. doi: 10.1016/j.tibtech.2019.02.007. Epub 2019 Mar 18. Trends Biotechnol. 2019. PMID: 30898338 Review.
An optimized genome-wide, virus-free CRISPR screen for mammalian cells.
Xiong K, Karottki KJC, Hefzi H, Li S, Grav LM, Li S, Spahn P, Lee JS, Ventina I, Lee GM, Lewis NE, Kildegaard HF, Pedersen LE. Xiong K, et al. Among authors: lee gm, lee js. Cell Rep Methods. 2021 Aug 23;1(4):100062. doi: 10.1016/j.crmeth.2021.100062. Epub 2021 Aug 4. Cell Rep Methods. 2021. PMID: 34935002 Free PMC article.
701 results