Restoration of serine protease-inhibitor interaction by protein engineering.
Madison EL, Goldsmith EJ, Gething MJ, Sambrook JF, Gerard RD.
Madison EL, et al. Among authors: goldsmith ej.
J Biol Chem. 1990 Dec 15;265(35):21423-6.
J Biol Chem. 1990.
PMID: 2123870
Free article.
We have previously demonstrated that mutation of arginine 304 of t-PA to a glutamic acid residue drastically reduces the rate of interaction between the enzyme and its suicide substrate, PAI-1, without affecting the reactivity of the enzyme toward its normal substrate, plasminoge …
We have previously demonstrated that mutation of arginine 304 of t-PA to a glutamic acid residue drastically reduces the rate of interaction …