[Study of the structure-function organization of the variola virus genome. IV. Sequencing and analysis of the nucleotide sequence of the right terminus of the India-1967 strain genome]

V M Blinov; A V Totmenin; S M Resenchuk; L V Olenina; V E Chizhikov; A A Kolykhalov; I V Frolov; V V Gutorov; S G Pozdniakov; V N Krasnykh

[Study of the structure-function organization of the variola virus genome. IV. Sequencing and analysis of the nucleotide sequence of the right terminus of the India-1967 strain genome]

Mol Biol (Mosk). 1995 Jul-Aug;29(4):772-89.

[Article in Russian]

Authors

V M Blinov, A V Totmenin, S M Resenchuk, L V Olenina, V E Chizhikov, A A Kolykhalov, I V Frolov, V V Gutorov, S G Pozdniakov, V N Krasnykh, et al.

PMID: 7476944

Abstract

Sequencing and computer analysis of the variola major virus strain India-1967 (VAR-IND) genome segment (53,018 bp) from the right terminal region have been carried out. Fifty nine potential open reading frames (ORFs) of over 60 amino acid residues have been identified. Structure-function organization of VAR-IND DNA segment under study was compared with the previously reported sequences from the analogous genomic regions of vaccinia virus strains Copenhagen (VAC-COP) and Western Reserve (VAC-WR) and variola virus strain Harvey (VAR-HAR). Multiple distinctions in the genetic map of VAR-IND from VAC-COP and VAC-WR have been revealed along with the high similarity to the corresponding VAR-HAR segment. Possible functions of the predicted viral proteins and the effect of their differences on the features of orthopoxviruses are discussed.

Publication types

English Abstract

MeSH terms

Amino Acid Sequence
Animals
DNA Ligases / genetics
DNA, Viral
Genome, Viral*
Molecular Sequence Data
Open Reading Frames
Plasmids
Receptors, Cytokine / genetics
Sequence Homology, Amino Acid
Species Specificity
Structure-Activity Relationship
Thymidine Kinase / genetics
Variola virus / genetics*

Substances

DNA, Viral
Receptors, Cytokine
Thymidine Kinase
DNA Ligases