Kill two birds with one stone: colorimetric/fluorescence immunosensor based on Au@Pt nanozyme for sensitive detection of imidacloprid

Mikrochim Acta. 2024 Oct 1;191(10):637. doi: 10.1007/s00604-024-06718-1.

Abstract

Gold-platinum (Au@Pt) nanozymes with high catalytic activity and stability were designed to improve the stability of the enzyme-linked immunosorbent assay (ELISA), and a two-mode signal output was used to enhance the sensitivity and confidence of the assay. This study reports the two-mode signal output based on Au@Pt nanozyme to catalyzed 3,3',5,5'-tetramethylbenzidine (TMB) reaction. Oxidized 3,3',5,5'-tetramethylbenzidine (ox-TMB) has wide absorption spectrum, providing excellent optical density capabilities and fluorescence quenching. The detection limits of imidacloprid were 0.88 μg/L and 1.14 μg/L in colorimetric and fluorescence modes, respectively. Multiple-mode strategy improves detection accuracy, increases the confidence of experimental results, and broadens detection modes. Two modes can meet the requirements of accurate and flexible multi-mode sensing in different application situations.

Keywords: Au@Pt; Colorimetric detection; Fluorescence; Imidacloprid; Immunoassay.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzidines* / chemistry
  • Biosensing Techniques / methods
  • Catalysis
  • Colorimetry* / methods
  • Gold* / chemistry
  • Insecticides / analysis
  • Limit of Detection*
  • Metal Nanoparticles / chemistry
  • Neonicotinoids* / analysis
  • Nitro Compounds* / analysis
  • Nitro Compounds* / chemistry
  • Platinum* / chemistry
  • Spectrometry, Fluorescence / methods

Substances

  • Neonicotinoids
  • imidacloprid
  • Nitro Compounds
  • Platinum
  • Gold
  • Benzidines
  • 3,3',5,5'-tetramethylbenzidine
  • Insecticides