Engineering GPCR signaling pathways with RASSLs

Nat Methods. 2008 Aug;5(8):673-8. doi: 10.1038/nmeth.1232.

Abstract

We are creating families of designer G protein-coupled receptors (GPCRs) to allow for precise spatiotemporal control of GPCR signaling in vivo. These engineered GPCRs, called receptors activated solely by synthetic ligands (RASSLs), are unresponsive to endogenous ligands but can be activated by nanomolar concentrations of pharmacologically inert, drug-like small molecules. Currently, RASSLs exist for the three major GPCR signaling pathways (G(s), G(i) and G(q)). We review these advances here to facilitate the use of these powerful and diverse tools.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Animals
  • Evolution, Molecular
  • Humans
  • Ligands
  • Protein Binding
  • Protein Engineering / methods*
  • Receptors, G-Protein-Coupled / analysis*
  • Receptors, G-Protein-Coupled / chemistry
  • Receptors, G-Protein-Coupled / genetics
  • Receptors, G-Protein-Coupled / metabolism*
  • Signal Transduction*

Substances

  • Ligands
  • Receptors, G-Protein-Coupled