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Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.
Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F. Konermann S, et al. Nature. 2015 Jan 29;517(7536):583-8. doi: 10.1038/nature14136. Epub 2014 Dec 10. Nature. 2015. PMID: 25494202 Free PMC article.
High-throughput functional genomics using CRISPR-Cas9.
Shalem O, Sanjana NE, Zhang F. Shalem O, et al. Nat Rev Genet. 2015 May;16(5):299-311. doi: 10.1038/nrg3899. Epub 2015 Apr 9. Nat Rev Genet. 2015. PMID: 25854182 Free PMC article. Review.
Genome editing using Cas9 nickases.
Trevino AE, Zhang F. Trevino AE, et al. Methods Enzymol. 2014;546:161-74. doi: 10.1016/B978-0-12-801185-0.00008-8. Methods Enzymol. 2014. PMID: 25398340