Abstract
Tn551 inactivation of femA, a factor involved in methicillin resistance of Staphylococcus aureus, caused the production of peptidoglycan in which the fraction of monoglycyl- and serine-containing muropeptides was increased at the expense of pentaglycyl muropeptides. femA mutants have a specific block in the biosynthesis of pentaglycine cross bridges after the addition of the first glycine residue.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Cell Wall / chemistry*
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Cell Wall / metabolism
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Genes, Bacterial / genetics
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Glycine / analysis
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Molecular Sequence Data
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Muramic Acids / chemistry*
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Muramic Acids / metabolism
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Mutagenesis, Insertional
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Oligopeptides / chemistry*
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Oligopeptides / metabolism
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Peptidoglycan / chemistry*
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Peptidoglycan / metabolism
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Protein Conformation
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Serine / analysis
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Staphylococcus aureus / chemistry*
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Staphylococcus aureus / genetics
Substances
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Muramic Acids
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Oligopeptides
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Peptidoglycan
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Serine
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Glycine