Dr. Subhasree Ray

Highlights

A Polyunsaturated fatty acids ketogenic diet was developed using unsaturated fatty acids with an omega 3: omega 6 ratio of 1:2.8.

The mean seizure reduction was 71.1 %, with no significant difference (p=0.488) between the two ketogenic diets.

Mean diet compliance rate was 78.3 %, with a significant linear trend between a higher compliance linked to greater seizure reduction (p = 0.042, Z=4.039).

Nausea (p=0.033) and vomiting (p=0.014) occurred more in the… Show more

Highlights

A Polyunsaturated fatty acids ketogenic diet was developed using unsaturated fatty acids with an omega 3: omega 6 ratio of 1:2.8.

The mean seizure reduction was 71.1 %, with no significant difference (p=0.488) between the two ketogenic diets.

Mean diet compliance rate was 78.3 %, with a significant linear trend between a higher compliance linked to greater seizure reduction (p = 0.042, Z=4.039).

Nausea (p=0.033) and vomiting (p=0.014) occurred more in the polyunsaturated group than in the classical ketogenic diet group.

The classical ketogenic diet remained preferred for managing refractory epilepsy in children, showing better compliance, tolerability, and fewer adverse effects. Show less

Blue foods are crucial for global food security and achieving the SDGs. By promoting sustainable aquaculture and responsible fishing, we can create a resilient food system for the future.

We addressed three research questions in this context: (a) Are all plant-based foods equal in terms of health and environmental benefits? (b) Do these products meet acceptance, accessibility, and affordability requirements? (c) Does this process omit the immense nutritional benefit of other well-documented healthful dietary patterns such as the Mediterranean diet? We anticipate that the answers will supply valuable information to a broad range of stakeholders, including the public, global food… Show more

We addressed three research questions in this context: (a) Are all plant-based foods equal in terms of health and environmental benefits? (b) Do these products meet acceptance, accessibility, and affordability requirements? (c) Does this process omit the immense nutritional benefit of other well-documented healthful dietary patterns such as the Mediterranean diet? We anticipate that the answers will supply valuable information to a broad range of stakeholders, including the public, global food production institutes, global nutrition bodies and researchers. Consequently, a well-rounded, economical, whole food-centred dietary approach can be formulated that will benefit us, our planet, and future generations. Show less

This brief makes a case for greater multisectoral investments in ECD (such as health education, nutrition, and child protection) and coherent policy responses. It uses India as a proof of concept with global potential by (1) understanding multidimensional deprivations among children under six; (2) tracking investments in ECD; and (3) ensuring sustainable access to nutrition and early learning opportunities in a ‘business not as usual’ manner.

Climate change, conflicts, and various crises have exposed the vulnerabilities of global food systems. Acute food insecurity and undernourishment have become more prevalent in recent times, and the imperative is to build more robust and sustainable food systems that do not adversely impact the environment. The COVID-19 pandemic has only underlined the importance of food security in times of crises. This brief explores the promise of climate-smart agriculture in combating climate change and… Show more

Climate change, conflicts, and various crises have exposed the vulnerabilities of global food systems. Acute food insecurity and undernourishment have become more prevalent in recent times, and the imperative is to build more robust and sustainable food systems that do not adversely impact the environment. The COVID-19 pandemic has only underlined the importance of food security in times of crises. This brief explores the promise of climate-smart agriculture in combating climate change and global food insecurity, and in nurturing sustainable food systems. Show less

It’s been six months since the World Health Organization has declared COVID 19 pandemic. COVID 19 is defining the global health crisis of our time and one of the major threats posed to the world since World War II. Worldwide, scientists are racing for a safe vaccine or medicine. Amidst this uncertainty, ‘immunity’ boosting’ is a trending topic in India and other parts of the world. The concept of immunityboosting is scientifically misled and commoditized during the pandemic by enchasing public… Show more

It’s been six months since the World Health Organization has declared COVID 19 pandemic. COVID 19 is defining the global health crisis of our time and one of the major threats posed to the world since World War II. Worldwide, scientists are racing for a safe vaccine or medicine. Amidst this uncertainty, ‘immunity’ boosting’ is a trending topic in India and other parts of the world. The concept of immunityboosting is scientifically misled and commoditized during the pandemic by enchasing public fear to contract the virus. By definition, immunity-boosting is a long-term procedure that cannot be achieved overnight by eating a single food or supplement. Strong immunity is an outcome of a holistic lifestyle that consists of healthy eating, physical activity, sound mental health and adequate sleep. Show less

Ketogenic Diet (KD) is a high-fat, low carbohydrate and normal or high protein diet at a ratio of 4:1 (Fat: Carbohydrate+Protein), predominantly used in refractory epilepsy. The fat used in classical ketogenic diet are predominantly saturated fatty acids. Poly-Unsaturated Fatty Acid Ketogenic Diet (PUFAKD) is composed of high polyunsaturated fatty acids (omega-6 and omega-3 at 2.8:1 ratio), optimum protein and low carbohydrate. A patient, after failure of 3 consecutive anti-epileptic drugs… Show more

Ketogenic Diet (KD) is a high-fat, low carbohydrate and normal or high protein diet at a ratio of 4:1 (Fat: Carbohydrate+Protein), predominantly used in refractory epilepsy. The fat used in classical ketogenic diet are predominantly saturated fatty acids. Poly-Unsaturated Fatty Acid Ketogenic Diet (PUFAKD) is composed of high polyunsaturated fatty acids (omega-6 and omega-3 at 2.8:1 ratio), optimum protein and low carbohydrate. A patient, after failure of 3 consecutive anti-epileptic drugs consulted the neurologist at an epilepsy management clinic and diagnosed as a case of refractory epilepsy. In the current case study, the patient (27 months old girl) was experiencing generalized tonic-clonic seizures. She was advised PUFAKD for 6 months to control the seizures and the progress was monitored during each monthly follow up. The PUFAKD intervention showed 50%, 65% and 80% seizure reduction at 3rd month, 4th month and 6th month respectively. The Anti-epileptic drugs (AEDs) were reduced from 3 to 2 after 6 months of intervention. The patient has shown 100% tolerability to the diet with no side effects. The present case study demonstrates promising role of PUFAKD in controlling refractory epilepsy.
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The case report describes one paediatric patient with refractory status epilepticus treated with the classical ketogenic diet. A patient diagnosed with refractory status epilepticus visited our clinic and was placed on the classical ketogenic diet after assessing biochemical-electrochemical parameters. The treatment was followed for a minimum of six months. The patient had an 85% seizure reduction, improved cognition and sound behavioural aspect after the treatment of six months. There was no… Show more

The case report describes one paediatric patient with refractory status epilepticus treated with the classical ketogenic diet. A patient diagnosed with refractory status epilepticus visited our clinic and was placed on the classical ketogenic diet after assessing biochemical-electrochemical parameters. The treatment was followed for a minimum of six months. The patient had an 85% seizure reduction, improved cognition and sound behavioural aspect after the treatment of six months. There was no reported evidence of side effect, commonly related to KD. The patient has shown good tolerability to the diet. The quality of life of the patient and family improved significantly. The number of antiepileptic drugs was reduced. The ketogenic diet is an effective and well-tolerated alternative treatment option for patients with refractory status epilepticus and should be considered as an effective course of treatment worldwide for treating refractory epileptic cases. Show less

The combined experimental and epidemiological evidence has led to designation of aflatoxins as human carcinogens according to International Agency for Cancer Research criteria. Mutation of p53 gene is found as a key factor in hepatocellular carcinoma (HCC), the most common form of liver cancer which is prominent in aflatoxin affected population. The site specific point mutation by Aflatoxin B1 (AFB1) generated epoxide, includes transversion of Guanine (G) to Thymine (T) in the 3rd position of… Show more

The combined experimental and epidemiological evidence has led to designation of aflatoxins as human carcinogens according to International Agency for Cancer Research criteria. Mutation of p53 gene is found as a key factor in hepatocellular carcinoma (HCC), the most common form of liver cancer which is prominent in aflatoxin affected population. The site specific point mutation by Aflatoxin B1 (AFB1) generated epoxide, includes transversion of Guanine (G) to Thymine (T) in the 3rd position of 249 codon of p53 gene by producing highly mutagenic AFB1-FAPY adduct. According to experimental studies nutritional antioxidants like vitamin A, Vitamin C, Vitamin E, chlorophyllin and fermented probiotics are already proven to be effective in scavenging this highly negative epoxide molecule to prevent aflatoxin induced hepatic carcinogenicity. In this context our perspective study tries to see the scope of green tea polyphenolic compound Epigallocathechingallate (EGCG) as an antioxidant-anticancerous molecule for restricting tumor cell production and promotion in aflatoxicity by considering fundamental knowledge of Nutritional Science, Bioinformatics and Genetics. This biochemical and genetic analysis based study focuses on future perspective of genetic trait specific personalized recommended value of green tea extract which can be formulated, quantified and prescribed in the prevention of Aflatoxin induced HCC after evaluating all side effect possibilities of EGCG and other polyphenols. The perspective study thus concludes with a vision for a paradigm shift in disease prevention strategy based on consuming specific-purified phenolic compound containing green tea to strengthen the existing knowledge of Food toxicological research Show less

Introduction: OJUS Medical Institute in collaboration with the Women & Child Development department of Nashik District, Maharashtra conducted a target-oriented pilot project study to see the improvement in body weight gain of SAM children, supplemented with a nutritionally enriched diet and micronutrients. The Egg-DOT project is a target vertical intervention study that involved both Anganwadi workers and Community health workers to bring a fruitful result by working hand-in-hand. Rationale:… Show more

Introduction: OJUS Medical Institute in collaboration with the Women & Child Development department of Nashik District, Maharashtra conducted a target-oriented pilot project study to see the improvement in body weight gain of SAM children, supplemented with a nutritionally enriched diet and micronutrients. The Egg-DOT project is a target vertical intervention study that involved both Anganwadi workers and Community health workers to bring a fruitful result by working hand-in-hand. Rationale: ICDS is one of the best supplementary nutrition programs to address and eliminate malnutrition in the country. To make it more comprehensive and result-oriented the pilot project study is formulated and executed to see if the addition in the existing system could eradicate malnutrition in an effective manner and strengthen the Govt. health machinery. Objective: The study aimed at working along with the Govt. to reduce severity of malnutrition. It shouted for a healthy public-private relationship to bring optimum results from the existing Govt. project in reducing the burden of malnutrition, spreading health education and behavioral modification in the community members by adopting a systematic micronutrient-diet-health education intervention strategy. Materials & methods: Along with existing nutritional intervention, a small modification in diet is introduced to fulfill the deficit of 300 Kcal approximately. Good quality fat and protein are added to the Anganwadi meal with daily micronutrient supplementation. The supplementation is continued for 30 days in 25 SAM children of 3 to 5 years. The baseline and end line bodyweight measurements are taken and compared to see the improvement. Result: After 30 days of intervention the supplemented SAM children showed statistically significant increased body weight (P<0.01) with an overall healthy nutritional status..... Show less

HIV infection results in gastro-intestinal tract damage, microbial translocation and immune
activation, which are associated with potential loss of CD4 + T cell count and persistent diarrhea. Increased morbidity and mortality of Anti-Retroviral Vaccine (ARV) treated individuals is associated with these dysfunctions. To enhance Gastro-Intestinal (GI) physiology of ARV treated-HIV infected population functional probiotic food groups’ revealed great efficacy. Probiotics are food with live… Show more

HIV infection results in gastro-intestinal tract damage, microbial translocation and immune
activation, which are associated with potential loss of CD4 + T cell count and persistent diarrhea. Increased morbidity and mortality of Anti-Retroviral Vaccine (ARV) treated individuals is associated with these dysfunctions. To enhance Gastro-Intestinal (GI) physiology of ARV treated-HIV infected population functional probiotic food groups’ revealed great efficacy. Probiotics are food with live bacterial culture, sufficient amount of which can confer great benefit to gut health and thus exert positive health benefits. Prebiotics are fermented ingredients that increase both activity and composition of GI microflora. Synbiotics are combination of probiotics and prebiotics. The current short systematic review aimed to study the use of probiotics, prebiotics and synbiotics in recovering immunological parameter in HIV infected population by reducing microbial translocation and increasing CD4 cell numbers. The findings demonstrated significant increment of CD4 cell level when experimented with these food groups. The challenge is to ensure probiotics in daily diet of HIV infected population with recommended dosage. Nutritional education should be accelerated to promote a cup of yogurt as a great addition to the gut care regimen for HIV infected population.

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Diet is a key factor in determining genomic stability is more important than previously imagined because it impacts on all relevant pathways like exposure to dietary carcinogens, DNA repair, DNA synthesis, epigenetic damage and apoptosis. Recent research focuses into how a single micronutrient deficiency is leading to genomic instability and development of degenerative diseases in various stages of life.The study aimed at finding the nutrigenomic mechanism of how a marginal deficiency of any… Show more

Diet is a key factor in determining genomic stability is more important than previously imagined because it impacts on all relevant pathways like exposure to dietary carcinogens, DNA repair, DNA synthesis, epigenetic damage and apoptosis. Recent research focuses into how a single micronutrient deficiency is leading to genomic instability and development of degenerative diseases in various stages of life.The study aimed at finding the nutrigenomic mechanism of how a marginal deficiency of any single micronutrient is interrupting in DNA repairing, methylation and synthesis by taking nutrient-nutrient and nutrient-gene interaction into consideration. It also focuses on how recommended dietary allowance is important in achieving DNA integrity and genome stability to prevent degenerative diseases.Exhaustive review of research papers in genome health nutrigenomicsis involved in this study to explore, assimilate and analyze data to understand the importance of micronutrient in maintaining methylation of CpG sequence and preventing DNA oxidation or uracil misincorporation in DNA to stop disease occurrence in individuals.The study finds a direct link between micronutrient deficiency and increased epigenomic damage, resulting into elevated risk for adverse health outcomes during various stages of life like infertility, tumor development and cancer. The overview study concludes with a vision for a paradigm shift in disease prevention strategy based on diagnosis and micro-nutritional intervention of genome or epigenome damage on an individual basis, i.e. personalized prevention of degenerative diseases in genome health clinic. Show less

Abstract: Botulinum toxin (BoNT), being the most poisonous substance poses a great threat to global public health as a deadly bio-weapon. BoNT/A, BoNT/B and BoNT/E serotype toxicity causes flaccid paralysis in humans by blocking neurotransmitter activity. Thearubigin, a black tea extract is found to be effective in neutralizing BoNT when experimented with animal models. This hypothesis opens a new frontier of Biological Science to understand the antitoxin property of thearubigin and apply the… Show more

Abstract: Botulinum toxin (BoNT), being the most poisonous substance poses a great threat to global public health as a deadly bio-weapon. BoNT/A, BoNT/B and BoNT/E serotype toxicity causes flaccid paralysis in humans by blocking neurotransmitter activity. Thearubigin, a black tea extract is found to be effective in neutralizing BoNT when experimented with animal models. This hypothesis opens a new frontier of Biological Science to understand the antitoxin property of thearubigin and apply the same on large scale to prevent BoNT neurotoxicity. Individualized dosage recommendation of thearubigin extract thus represents a natural antitoxin therapy in managing severe botulism in humans. Show less

Personalized Nutrition means in practice, adapting food to individual needs, depending on the host’s genome, this calls for an emerging field of nutrigenomic approach in order to build the tools for individualized diet, health maintenance, and disease prevention. Based on this principle, breast milk is now being analyzed, modified and administered in smaller infants to provide them personalized diet, ensuring the premature infants are receiving correct amounts of nutrients they need to thrive… Show more

Personalized Nutrition means in practice, adapting food to individual needs, depending on the host’s genome, this calls for an emerging field of nutrigenomic approach in order to build the tools for individualized diet, health maintenance, and disease prevention. Based on this principle, breast milk is now being analyzed, modified and administered in smaller infants to provide them personalized diet, ensuring the premature infants are receiving correct amounts of nutrients they need to thrive. In the past, all milk was fortified to the same and it was ‘one-size-fits-all’. Now, nutrigenomics is moving towards having the ability to personalize each mother’s milk to give her baby the precise nutrition he needs by stressing upon nutrition and interaction of three health-relevant genomes in perspective, namely the food, the gut microbial, and the human host genome in context of individualized nutrition and optimum health. Show less