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Research Article Free access | 10.1172/JCI107273
Department of Medicine and Regional Primate Research Center, University of Washington 98195
Donner Laboratory, Lawrence Radiation Laboratory, University of California, Berkeley, California 94720
Institute for Nutrition Research, School of Medicine, University of Oslo, Oslo 3, Norway
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Department of Medicine and Regional Primate Research Center, University of Washington 98195
Donner Laboratory, Lawrence Radiation Laboratory, University of California, Berkeley, California 94720
Institute for Nutrition Research, School of Medicine, University of Oslo, Oslo 3, Norway
Find articles by Nichols, A. in: JCI | PubMed | Google Scholar
Department of Medicine and Regional Primate Research Center, University of Washington 98195
Donner Laboratory, Lawrence Radiation Laboratory, University of California, Berkeley, California 94720
Institute for Nutrition Research, School of Medicine, University of Oslo, Oslo 3, Norway
Find articles by Norum, K. in: JCI | PubMed | Google Scholar
Department of Medicine and Regional Primate Research Center, University of Washington 98195
Donner Laboratory, Lawrence Radiation Laboratory, University of California, Berkeley, California 94720
Institute for Nutrition Research, School of Medicine, University of Oslo, Oslo 3, Norway
Find articles by King, W. in: JCI | PubMed | Google Scholar
Department of Medicine and Regional Primate Research Center, University of Washington 98195
Donner Laboratory, Lawrence Radiation Laboratory, University of California, Berkeley, California 94720
Institute for Nutrition Research, School of Medicine, University of Oslo, Oslo 3, Norway
Find articles by Forte, T. in: JCI | PubMed | Google Scholar
Published May 1, 1973 - More info
Plasma lipoproteins of d<1.006 g/ml, d 1.006-1.019 g/ml, and d 1.019-1.063 g/ml from patients with familial lecithin:cholesterol acyltransferase deficiency yielded abnormal subfractions upon being separately filtered through 2% agarose gel. A subfraction that emerged with the void volume and contained unusually large amounts of unesterified cholesterol and phosphatidylcholine was present in each lipoprotein group, and in each group this subfraction was less prominent in the nonlipemic plasma of one patient than in the lipemic plasma of other patients. A subfraction containing smaller lipoproteins also was present in each lipoprotein group. These lipoproteins were of the same size as normal lipoproteins of the corresponding density, but contained abnormally small amounts of cholesteryl ester. The lipoproteins of 1.019-1.063 g/ml contained abnormal components of intermediate molecular weight as well as large and small abnormal components similar to those described previously. The intermediate components were more prominent in the nonlipemic plasma but were easily recognized in the hyperlipemic plasma as a peak of Sf 20-30 in the analytical ultracentrifuge. Also they could be recognized, upon electron microscopy of the lipoproteins of d 1.019-1.063 g/ml, as particles 340-1000 Å in diameter.
The data suggest that related large, abnormal particles pervade the patients' very low and low density lipoproteins, and that the large particles are affected by, but are not dependent on, the lipemia that frequently accompanies the disease. The smaller very low and low density lipoproteins appear to be counterparts of lipoproteins present in normal plasma. Their abnormal composition is compatible with the possibility that lecithin:cholesterol acyltransferase normally decreases the triglyceride and phosphatidylcholine and increases the cholesteryl ester of very low density and low density plasma lipoproteins in vivo.
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