Abstract
The bacterial outer membrane proteins OmpA and OmpX were modified in such a manner that they yielded bulky crystals diffracting X-rays isotropically beyond 2 A resolution and permitting detailed structural analyses. The procedure involved semi-directed mutagenesis, mass production into inclusion bodies, and (re)naturation therefrom; it should be applicable for a broader range of membrane proteins.
MeSH terms
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Bacterial Outer Membrane Proteins / biosynthesis
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Bacterial Outer Membrane Proteins / chemistry*
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Bacterial Outer Membrane Proteins / genetics
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Crystallization
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Crystallography, X-Ray
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Escherichia coli / chemistry*
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Escherichia coli / genetics
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Escherichia coli Proteins*
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Hydrolases*
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Inclusion Bodies / metabolism
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Mutagenesis
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Protein Denaturation
Substances
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Bacterial Outer Membrane Proteins
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Escherichia coli Proteins
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OmpX protein, E coli
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OMPA outer membrane proteins
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Hydrolases