Abstract
We have isolated a cDNA (bv7-3) encoding a member of the Na+,Cl(-)-dependent transporter family and its short splicing variant (bv7-3s) by screening a bovine retina cDNA library. Sequence analysis revealed that bv7-3 encodes a protein of 729 amino acids and is a bovine homologue of the rat orphan transporter v7-3-2. bv7-3s contains 265 amino acids, sharing 252 N-terminal amino acids with bv7-3. Both mRNAs for bv7-3 and bv7-3s were detected in nervous system by Northern blot analysis. In immunofluorescence analysis in transfected HEK 293T cells, myc-tagged bv7-3 was mainly detected on the plasma membrane, whereas myc-tagged bv7-3s showed a pattern of intracellular membrane staining.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alternative Splicing*
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Amino Acid Sequence
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Animals
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Base Sequence
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Blotting, Northern
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Brain Chemistry
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Carrier Proteins / analysis
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Carrier Proteins / chemistry
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Carrier Proteins / classification
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Carrier Proteins / genetics*
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Cattle
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Cell Line
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Cell Membrane / chemistry
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Cloning, Molecular*
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Fluorescent Antibody Technique
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Gene Expression
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Gene Library
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Humans
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Ion Pumps*
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Membrane Transport Proteins*
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Molecular Sequence Data
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Nerve Tissue Proteins / genetics
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Plasma Membrane Neurotransmitter Transport Proteins
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Rats
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Recombinant Fusion Proteins / analysis
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Retina / chemistry
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Sequence Homology, Amino Acid
Substances
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Bv7-3 protein, Bos taurus
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Carrier Proteins
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Ion Pumps
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Membrane Transport Proteins
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Nerve Tissue Proteins
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Plasma Membrane Neurotransmitter Transport Proteins
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Recombinant Fusion Proteins
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Slc6a17 protein, rat