Amplification products of the HUMFIBRA locus were analysed in a population from Northern Poland using capillary electrophoresis. The most frequent alleles were 21, 22 and 20 with a frequencies of 0.201, 0.179 and 0.167 respectively, and five rare variants were observed with frequencies less than 0.015. A homogeneous allele distribution was found between Polish and Dutch populations and significant differences between Poles and Italians. The high discrimination power (0.963), polymorphic information content (0.849) and heterozygosity confirm the usefulness of HUMFIBRA locus for forensic identification purposes. The application of capillary electrophoresis offers the possibility of precise identification of rare variants.