Abstract
Conductin or Axil, an Axin homolog, plays an important role in the regulation of beta-catenin stability in the Wnt signaling pathway. To facilitate the molecular analysis of the human gene, we isolated the human homolog, AXIN2. The cDNA contains a 2529-bp open reading frame and encodes a putative protein of 843 amino acids. Compared with rat and mouse homologs, AXIN2 shows an overall 89% amino acid identity. Several functional domains in this protein are highly conserved including the GRS (95.9%), GSK-3beta (96.3%), Dsh (98%), and beta-catenin (89.9%) domains. Radiation hybrid mapping localized the AXIN2 gene to human chromosome 17q23-q24, a region that shows frequent loss of heterozygosity in breast cancer, neuroblastoma, and other tumors. Human AXIN2 is thus a very strong candidate involved in multiple tumor types.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Adaptor Proteins, Signal Transducing
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Amino Acid Sequence
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Animals
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Axin Protein
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Carrier Proteins*
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Chromosomes, Human, Pair 17 / genetics*
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Cloning, Molecular
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Cytoskeletal Proteins / genetics*
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DNA Primers
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Humans
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Molecular Sequence Data
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Proteins / genetics*
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Proto-Oncogene Proteins / genetics
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Rats
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Repressor Proteins*
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Sequence Homology, Amino Acid
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Signal Transduction
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Trans-Activators*
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Wnt Proteins
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Zebrafish Proteins*
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beta Catenin
Substances
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AXIN2 protein, human
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Adaptor Proteins, Signal Transducing
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Axin Protein
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Axin2 protein, rat
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CTNNB1 protein, human
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Carrier Proteins
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Ctnnb1 protein, rat
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Cytoskeletal Proteins
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DNA Primers
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Proteins
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Proto-Oncogene Proteins
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Repressor Proteins
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Trans-Activators
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Wnt Proteins
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Zebrafish Proteins
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beta Catenin