Abstract
Salmon insulin-like growth factor-I (sIGF-I) expression is, as in mammals, induced by growth hormone (GH). To elucidate the mechanism by which GH stimulates the transcription of the IGF-I gene, we transiently transfected Hep3B cells expressing the rat GH receptor with a sIGF-I promoter-luciferase reporter construct. Activation of the construct by GH added to the medium of the transfected cells was observed when two specific transcription factors, STAT5 and HNF-1alpha, were simultaneously overexpressed in these cells. This finding demonstrates for the first time a GH-dependent activation of an IGF-I promoter construct in an immortalized laboratory cell line.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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DNA-Binding Proteins / genetics*
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Gene Expression Regulation / genetics*
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Genes, Reporter / genetics
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Growth Hormone / pharmacology*
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Hepatocyte Nuclear Factor 1
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Hepatocyte Nuclear Factor 1-alpha
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Hepatocyte Nuclear Factor 1-beta
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Insulin-Like Growth Factor I / genetics*
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Milk Proteins*
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Nuclear Proteins*
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Promoter Regions, Genetic / genetics
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Receptors, Somatotropin / genetics
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STAT5 Transcription Factor
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Salmon / metabolism
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Trans-Activators / genetics*
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Transcription Factors / genetics*
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Transcriptional Activation / genetics
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Transfection / genetics
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Tumor Cells, Cultured
Substances
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DNA-Binding Proteins
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Hepatocyte Nuclear Factor 1-alpha
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Milk Proteins
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Nuclear Proteins
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Receptors, Somatotropin
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STAT5 Transcription Factor
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Trans-Activators
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Transcription Factors
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Hepatocyte Nuclear Factor 1
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Hepatocyte Nuclear Factor 1-beta
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Insulin-Like Growth Factor I
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Growth Hormone