Objective: To report the successful use of testicular sperm aspiration and intracytoplasmic sperm injection in the presence of an Escherichia coli-infected ejaculate that previously caused repeated embryo degeneration.
Design: Case report.
Setting: University medical center.
Patient(s): A 38-year-old woman who did not conceive for 6 years with repeated IVF attempts. Escherichia coli was isolated from both the oocyte culture dish and her male partner's ejaculate.
Intervention(s): Testicular sperm aspiration and intracytoplasmic sperm injection followed by ET.
Main outcome measure(s): Clinical outcome.
Result(s): Establishment of a pregnancy delivered at term.
Conclusion(s): Patients undergoing IVF treatment who have repeated embryo degeneration caused by bacterial infection originating in the ejaculate may be treated successfully with testicular sperm aspiration and intracytoplasmic sperm injection.