Abstract
Wnt/Wingless directs many cell fates during development. Wnt/Wingless signaling increases the amount of beta-catenin/Armadillo, which in turn activates gene transcription. Here the Drosophila protein D-Axin was shown to interact with Armadillo and D-APC. Mutation of d-axin resulted in the accumulation of cytoplasmic Armadillo and one of the Wingless target gene products, Distal-less. Ectopic expression of d-axin inhibited Wingless signaling. Hence, D-Axin negatively regulates Wingless signaling by down-regulating the level of Armadillo. These results establish the importance of the Axin family of proteins in Wnt/Wingless signaling in Drosophila.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing*
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Adenomatous Polyposis Coli Protein
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Animals
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Armadillo Domain Proteins
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Axin Protein
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Body Patterning
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Carrier Proteins / chemistry
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Carrier Proteins / genetics
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Carrier Proteins / metabolism*
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Chromosome Mapping
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Cytoplasm / metabolism
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Cytoskeletal Proteins / metabolism
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Down-Regulation
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Drosophila / embryology*
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Drosophila / genetics
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Drosophila / metabolism
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Drosophila Proteins*
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Embryo, Nonmammalian / metabolism
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Extremities / embryology
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Gene Expression Regulation, Developmental
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Genes, Insect
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism
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In Situ Hybridization
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Insect Proteins / genetics
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Insect Proteins / metabolism
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Molecular Sequence Data
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Mutation
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Phenotype
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Proteins / chemistry
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Proteins / genetics
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Proteins / metabolism*
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Proto-Oncogene Proteins / metabolism*
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Recombinant Fusion Proteins / metabolism
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Repressor Proteins*
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Signal Transduction*
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Trans-Activators*
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Transcription Factors*
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Wings, Animal / embryology
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Wings, Animal / metabolism
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Wnt1 Protein
Substances
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APC protein, Drosophila
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ARM protein, Drosophila
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Adaptor Proteins, Signal Transducing
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Adenomatous Polyposis Coli Protein
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Armadillo Domain Proteins
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Axin Protein
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Axn protein, Drosophila
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Carrier Proteins
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Cytoskeletal Proteins
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Drosophila Proteins
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Homeodomain Proteins
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Insect Proteins
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Proteins
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Proto-Oncogene Proteins
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Recombinant Fusion Proteins
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Repressor Proteins
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Trans-Activators
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Transcription Factors
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Wnt1 Protein
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distal-less protein, insect
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wg protein, Drosophila