Abstract
A previous study has shown that Sm37-5 is a major B cell epitope of Sm37-GAPDH. This epitope is highly antigenic in human infections and IgG antibody reactivity toward this determinant is associated with adolescent resistance to reinfection. This led us to test a synthetic peptide corresponding to Sm37-5, coupled to ovalbumin, as an anti-schistosome vaccine. Although mice injected with Sm37-5-OVA in Freund's adjuvant showed significant protection, immunization in aluminium hydroxide failed to induce protection. The adjuvant effect of cytokines (GM-CSF or IL-12) associated with the antigen on alum was investigated. With each of these two cytokines, significant reductions in the worm burden were obtained (32-38% with GM-CSF and 27% with IL-12, respectively). In addition, a reduction of the egg number trapped in the liver of immunized mice was also observed. Thus, protections were obtained with formulations that could potentially be used in humans.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / administration & dosage
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Adsorption
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Alum Compounds*
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Animals
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Antibodies, Helminth / immunology
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Antigens, Helminth / administration & dosage
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Antigens, Helminth / immunology
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Epitopes, B-Lymphocyte / immunology*
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Female
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Glyceraldehyde-3-Phosphate Dehydrogenases / immunology
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Granulocyte-Macrophage Colony-Stimulating Factor / administration & dosage
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Granulocyte-Macrophage Colony-Stimulating Factor / immunology*
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Immunity, Innate / immunology
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Immunoglobulin G / biosynthesis
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Injections, Intramuscular
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Interleukin-12 / administration & dosage
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Interleukin-12 / immunology*
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Mice
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Mice, Inbred CBA
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Molecular Weight
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Ovalbumin / administration & dosage
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Ovalbumin / immunology*
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Schistosoma mansoni / immunology*
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Schistosomiasis mansoni / immunology*
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Schistosomiasis mansoni / prevention & control
Substances
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Adjuvants, Immunologic
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Alum Compounds
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Antibodies, Helminth
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Antigens, Helminth
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Epitopes, B-Lymphocyte
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Immunoglobulin G
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Interleukin-12
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aluminum sulfate
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Granulocyte-Macrophage Colony-Stimulating Factor
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Ovalbumin
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Glyceraldehyde-3-Phosphate Dehydrogenases