Crystallization of two related lectins from the legume plant Dolichos biflorus

Acta Crystallogr D Biol Crystallogr. 1998 Nov 1;54(Pt 6 Pt 2):1446-9. doi: 10.1107/s0907444998004478.

Abstract

The seed lectin DBL and the related stem and leaves lectin DB58 of the tropical legume Dolichos biflorus were crystallized, as well as complexes of DBL with adenine and with GalNAc(alpha1-3)[Fuc(alpha1-2)]Gal. The different crystal forms of DBL diffract to about 2.8 A, while DB58 crystals diffract to 3.3 A.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ABO Blood-Group System
  • Adenine / metabolism
  • Carbohydrate Sequence
  • Crystallization
  • Crystallography, X-Ray
  • Lectins / chemistry*
  • Lectins / isolation & purification
  • Lectins / metabolism
  • Macromolecular Substances
  • Molecular Sequence Data
  • Oligosaccharides / metabolism
  • Oligosaccharides, Branched-Chain
  • Plant Lectins*
  • Receptors, Mitogen / metabolism
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / isolation & purification
  • Recombinant Fusion Proteins / metabolism

Substances

  • ABO Blood-Group System
  • DB58 lectin, Dolichos biflorus
  • Dolichos biflorus lectin receptor
  • Lectins
  • Macromolecular Substances
  • Oligosaccharides
  • Oligosaccharides, Branched-Chain
  • Plant Lectins
  • Receptors, Mitogen
  • Recombinant Fusion Proteins
  • blood group A trisaccharide
  • dolichos biflorus agglutinin
  • Adenine