Abstract
PhoB is the response regulator of the E. coli two-component signal transduction system for phosphate regulation. It is a transcription factor that activates more than 30 genes of the pho regulon. Crystals of the receiver domain of PhoB were obtained by applying the hanging-drop vapour-diffusion method. X-ray diffraction data have been collected using synchrotron radiation to 1.88 A resolution. The crystals belong to the orthorhombic space group P212121 with unit-cell constants a = 34.11, b = 60.42, c = 119.97 A. The Matthews parameter suggests that PhoB crystallizes with two molecules per asymmetric unit, suggesting that activating dimerization occurs in the crystal.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacterial Proteins / chemistry*
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Bacterial Proteins / genetics
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Bacterial Proteins / isolation & purification
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Crystallization
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Crystallography, X-Ray
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Escherichia coli / chemistry*
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Escherichia coli / genetics
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Membrane Proteins / chemistry
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Methyl-Accepting Chemotaxis Proteins
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Recombinant Fusion Proteins / chemistry
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Recombinant Fusion Proteins / isolation & purification
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Transcription Factors / chemistry*
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Transcription Factors / genetics
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Transcription Factors / isolation & purification
Substances
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Bacterial Proteins
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Membrane Proteins
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Methyl-Accepting Chemotaxis Proteins
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Recombinant Fusion Proteins
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Transcription Factors
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PhoB protein, Bacteria