Abstract
The region q13-15 of chromosome 12 contains SAS, CDK4, and MDM2 genes that are rearranged or amplified in a variety of human sarcomas. This study evaluated SAS gene amplification, and MDM2 and CDK4 protein expression in 20 tumor samples of central low-grade osteosarcoma (16 primary, 3 recurrences, 1 lung metastasis). SAS amplification was analyzed by quantitative polymerase chain reaction (PCR), while from the same paraffin-embedded samples, MDM2 and CDK4 protein expression was evaluated by immunohistochemistry. MDM2 and CDK4 proteins were found strongly expressed in 35% and 65%, respectively, of the samples. SAS was found amplified in 15% of the samples. These findings indicate that these genes may be involved in tumorigenesis and progression of low-grade osteosarcoma.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adolescent
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Adult
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Chromosomes, Human, Pair 12
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinases / genetics
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Cyclin-Dependent Kinases / metabolism*
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DNA, Neoplasm / isolation & purification
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Female
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Gene Expression
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Humans
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Immunohistochemistry
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Male
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Middle Aged
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Neoplasm Proteins / genetics
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Neoplasm Proteins / metabolism*
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Nuclear Proteins*
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Osteosarcoma / genetics
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Osteosarcoma / metabolism*
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Osteosarcoma / pathology
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Polymerase Chain Reaction
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / metabolism*
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Proto-Oncogene Proteins c-mdm2
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Tetraspanins
Substances
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DNA, Neoplasm
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Membrane Proteins
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Neoplasm Proteins
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Nuclear Proteins
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Proto-Oncogene Proteins
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TSPAN31 protein, human
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Tetraspanins
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MDM2 protein, human
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Proto-Oncogene Proteins c-mdm2
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CDK4 protein, human
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Cyclin-Dependent Kinase 4
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Cyclin-Dependent Kinases