Expression of tyrosine hydroxylase, calcitonin gene-related peptide, substance P and protein gene product 9.5 in mouse islets transplanted under the kidney capsule

Neuropeptides. 1998 Aug;32(4):307-18. doi: 10.1016/s0143-4179(98)90053-1.

Abstract

Pancreatic islets transplanted to the kidney of syngeneic mice were stained for calcitonin gene-related peptide (CGRP), substance P (SP), tyrosine hydroxylase (TH), acetylcholinesterase and the pan-neuronal marker, protein gene product 9.5 (PGP). Nerve fibers expressing TH-like immunoreactivity (TH-LI) and CGRP-LI were rare for 4 days but increased 2 (CGRP) or 6 (TH) weeks after transplantation. In 1-year-old grafts the CGRP-LI innervation resembled that in situ, while TH-LI and PGP-LI innervations were increased. SP-LI fibers remained rare throughout. Perikarya intrinsic to the islets did not show CGRP-LI or SP-LI. The results indicate a progressive ingrowth of sensory fibers into the grafts and that the TH-LI innervation becomes even more pronounced than in the pancreas. The post-transplantation reaction of islet intrinsic neurons does not involve CGRP and SP, contrasting with previous observations for vasoactive intestinal polypeptide.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcitonin Gene-Related Peptide / analysis*
  • Female
  • Immunohistochemistry
  • Islets of Langerhans / chemistry*
  • Islets of Langerhans / innervation
  • Islets of Langerhans Transplantation*
  • Kidney
  • Mice
  • Mice, Inbred C57BL
  • Nerve Fibers / chemistry
  • Neurons / chemistry
  • Substance P / analysis*
  • Thiolester Hydrolases / analysis*
  • Transplantation, Heterotopic
  • Tyrosine 3-Monooxygenase / analysis*
  • Ubiquitin Thiolesterase

Substances

  • Substance P
  • Tyrosine 3-Monooxygenase
  • Thiolester Hydrolases
  • Ubiquitin Thiolesterase
  • Calcitonin Gene-Related Peptide