Dexamethasone induces apoptosis in human T cell clones expressing low levels of Bcl-2

Cell Death Differ. 1999 Jan;6(1):79-86. doi: 10.1038/sj.cdd.4400461.

Abstract

Previous results of ours have demonstrated that the same clonotype can express both a sensitive and a resistant phenotype to Dex-mediated PCD induction depending on its cell cycle phase. In particular, we demonstrated that human T lymphocytes, arrested in the G0/G1 phase of the cell cycle, are susceptible, while proliferating T cells are resistant to Dex-mediated apoptosis. In this paper, we have further characterized the sensitive and resistant phenotypes and investigated whether a different expression of the apoptotic genes Fas, FasL, Bcl-2, Bcl-x and Bax is involved in the regulation of Dex-mediated apoptosis. The results show that the amount of Bcl-2 expression, that changes during cell cycle phases, determines susceptibility or resistance to apoptosis induced by Dex. In fact, undetectable expression of Bcl-2 in sensitive cells favors Dex-mediated apoptosis while high expression of Bcl-2 in proliferating cells counterbalances apoptosis induction. Moreover, the addition of exogenous IL-2, in the presence of Dex, fails to up-regulate Bcl-2 expression and to revert Dex-mediated apoptotic phenomena.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / genetics*
  • Cell Cycle / genetics
  • Cell Division / genetics
  • Clone Cells
  • DNA / analysis
  • Dexamethasone / pharmacology*
  • Fas Ligand Protein
  • Flow Cytometry
  • Gene Expression Regulation
  • Humans
  • Interleukin-2 / pharmacology
  • Membrane Glycoproteins / genetics
  • Phenotype
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins c-bcl-2 / genetics*
  • T-Lymphocytes / drug effects*
  • bcl-2-Associated X Protein
  • bcl-X Protein
  • fas Receptor / genetics

Substances

  • BAX protein, human
  • BCL2L1 protein, human
  • FASLG protein, human
  • Fas Ligand Protein
  • Interleukin-2
  • Membrane Glycoproteins
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • bcl-2-Associated X Protein
  • bcl-X Protein
  • fas Receptor
  • Dexamethasone
  • DNA