Abstract
A hexadecyltrimethylammonium bromide (CTAB) method for isolating fungal DNA from clinical samples, suitable for PCR amplification is described. Yeast and filamentous fungi DNA from clinical samples was amplified with primers complementary to the genes coding for rRNA, amplifying a 105 bp fragment and internal transcribed spacer primers amplifying fragments between 242 and 622 bp. The level of sensitivity was 10 +/- 5 yeast and 28 Aspergillus fumigatus CFU ml-1 of biological fluid.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Blood / microbiology*
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Cetrimonium
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Cetrimonium Compounds*
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Colony Count, Microbial
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DNA Primers
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DNA, Fungal / isolation & purification*
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Fungemia / microbiology*
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Genes, rRNA
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Humans
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Mitosporic Fungi / growth & development
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Mitosporic Fungi / isolation & purification
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Mycoses / microbiology
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Polymerase Chain Reaction / methods*
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RNA, Ribosomal / genetics
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Sensitivity and Specificity
Substances
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Cetrimonium Compounds
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DNA Primers
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DNA, Fungal
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RNA, Ribosomal
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Cetrimonium