Differential induction of interferon (IFN)-inducible protein 10 following differentiation of a monocyte, macrophage cell lineage is related to the changes of nuclear proteins bound to IFN stimulus response element and kappaB sites

Int J Mol Med. 1999 May;3(5):477-84. doi: 10.3892/ijmm.3.5.477.

Abstract

We examined chemokine gene expression following the differentiation of a monocyte, macrophage cell lineage. The human monoblastic cell line, U937 was differentiated to macrophages by the treatment with either phorbol 12-myristate 13-acetate (PMA), retinoic acid (RA), or vitamin D3 (VitD3). The gene expression of interferon (IFN)-inducible protein 10 (IP-10) (a CXC chemokine) was markedly augmented by the IFNgamma treatment in PMA- or RA-differentiated U937 cells, but only marginally in undifferentiated or VitD3-treated cells. In contrast, another inducible gene expression of monocyte chemotactic protein-1 (a CC chemokine) and the activation of the transcriptional factor (FcRFgamma) bound to the gamma response region were similarly or less abundantly induced by IFNgamma treatment in PMA- or RA-differentiated U937 cells, indicating that increased IP-10 mRNA induction was not due to the augmented ability of the cells to respond to the presence of IFNgamma. Increased expression of IFNgamma-induced IP-10 mRNA following the differentiation of U937 cells was mediated largely by augmented transcriptional activity of the gene and was related to differentiation-dependent changes of the proteins bound to IFN stimulus response element (ISRE) and kB sites, suggesting that these nuclear proteins may determine the IP-10 mRNA inducibility by IFNgamma.

MeSH terms

  • Base Sequence
  • Binding Sites / genetics
  • Cell Differentiation / drug effects
  • Chemokine CCL2 / genetics
  • Chemokine CXCL10
  • Chemokines, CXC / biosynthesis*
  • Chemokines, CXC / genetics*
  • DNA Primers / genetics
  • Gene Expression Regulation / drug effects
  • Humans
  • Interferon-gamma / pharmacology*
  • Macrophages / cytology
  • Macrophages / drug effects
  • Macrophages / metabolism
  • Monocytes / cytology
  • Monocytes / drug effects
  • Monocytes / metabolism
  • NF-kappa B / metabolism*
  • Nuclear Proteins / metabolism*
  • Protein Binding
  • RNA, Messenger / biosynthesis
  • RNA, Messenger / genetics
  • Recombinant Proteins
  • Tetradecanoylphorbol Acetate / pharmacology
  • Tretinoin / pharmacology
  • U937 Cells

Substances

  • Chemokine CCL2
  • Chemokine CXCL10
  • Chemokines, CXC
  • DNA Primers
  • NF-kappa B
  • Nuclear Proteins
  • RNA, Messenger
  • Recombinant Proteins
  • Tretinoin
  • Interferon-gamma
  • Tetradecanoylphorbol Acetate