Abstract
Arabidopsis thaliana BAC T07M07 encoding the abscisic acid-insensitive 4 (ABI4) locus has been sequenced completely. It contains a 95,713-bp insert and 24 predicted genes. Most putative genes were confirmed by gel-based RNA profiling and a cluster of ABA-regulated genes was identified. One of the 24 genes, designated PP2C5, encodes a putative protein phosphatase 2C. The encoded protein was expressed in Escherichia coli, and its enzyme activity in vitro was confirmed.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Abscisic Acid / metabolism
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Amino Acid Sequence
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Arabidopsis / genetics*
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Arabidopsis / metabolism
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Blotting, Northern / methods
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Chromosomes, Bacterial
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Cloning, Molecular / methods
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Gene Expression Regulation, Plant*
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Genes, Plant*
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Molecular Sequence Data
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Multigene Family / genetics*
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Phosphoprotein Phosphatases / genetics
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Protein Phosphatase 2
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Protein Phosphatase 2C
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Saccharomyces cerevisiae Proteins*
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Sequence Analysis, DNA
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Sequence Homology, Amino Acid
Substances
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Saccharomyces cerevisiae Proteins
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Abscisic Acid
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PTC1 protein, S cerevisiae
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Phosphoprotein Phosphatases
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Protein Phosphatase 2
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Protein Phosphatase 2C