Abstract
The activation of extracellular signal-regulated kinases 1 and 2 by insulin-like growth factor I in human osteosarcoma MG-63 cells was examined by Mono Q ion exchange chromatography of cell extracts and measurement of myelin basic protein kinase activity, and by immunoblotting of cell extracts with a phospho-specific extracellular signal-regulated kinase antibody. Extracellular signal-regulated kinase 1 appeared to be activated in resting cells and addition of insulin-like growth factor I resulted in the activation primarily of extracellular signal-regulated kinase 2. Extracellular signal-regulated kinase 2 was found in the nucleus after addition of insulin-like growth factor I.
MeSH terms
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Calcium-Calmodulin-Dependent Protein Kinases / metabolism*
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Cell Nucleus / enzymology
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Chromatography, High Pressure Liquid
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Fibroblasts / cytology
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Fibroblasts / enzymology
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Gene Expression Regulation, Enzymologic
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Gene Expression Regulation, Neoplastic
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Humans
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Insulin-Like Growth Factor I / physiology*
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3
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Mitogen-Activated Protein Kinases*
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Osteosarcoma*
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Receptor, IGF Type 1 / genetics
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Signal Transduction / physiology*
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Tumor Cells, Cultured / enzymology
Substances
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Insulin-Like Growth Factor I
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Receptor, IGF Type 1
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Calcium-Calmodulin-Dependent Protein Kinases
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Mitogen-Activated Protein Kinase 1
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Mitogen-Activated Protein Kinase 3
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Mitogen-Activated Protein Kinases