Abstract
We investigated the modulation of the expression and phosphatase activity of SHP-1 during granulocytic differentiation of human myeloid leukemia cell line, HL60 and t(15;17) positive APL cell line, HT93, in response to all-trans retinoic acid (ATRA) or dimethylsulfoxide (DMSO). ATRA induced differentiation in both cell lines which was associated with marked growth inhibition and S-phase reduction. On the other hand, DMSO induced it only in HL60 without obvious growth inhibition and S-phase reduction. The expression and phosphatase activity of SHP-1 were upregulated only when the 2 cell lines were differentiated to granulocytes. Furthermore, the changes were not dependent on the inducers or the growth inhibition. These findings suggest that SHP-1 is involved in common myeloid differentiation, and that upregulation of SHP-1 is not always related to myeloid cell growth.
MeSH terms
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Cell Cycle / drug effects
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Cell Differentiation / drug effects
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Cell Division / drug effects
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Dimethyl Sulfoxide / pharmacology
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Gene Expression Regulation, Enzymologic
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Granulocytes / cytology
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Granulocytes / enzymology*
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HL-60 Cells / cytology
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HL-60 Cells / enzymology*
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Humans
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Intracellular Signaling Peptides and Proteins
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Kinetics
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Leukemia, Promyelocytic, Acute / enzymology*
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Leukemia, Promyelocytic, Acute / pathology*
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Protein Tyrosine Phosphatase, Non-Receptor Type 11
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Protein Tyrosine Phosphatase, Non-Receptor Type 6
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Protein Tyrosine Phosphatases / genetics*
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Protein Tyrosine Phosphatases / metabolism*
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S Phase
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SH2 Domain-Containing Protein Tyrosine Phosphatases
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Time Factors
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Tretinoin / pharmacology
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Tumor Cells, Cultured
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src Homology Domains
Substances
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Intracellular Signaling Peptides and Proteins
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Tretinoin
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PTPN11 protein, human
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PTPN6 protein, human
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Protein Tyrosine Phosphatase, Non-Receptor Type 11
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Protein Tyrosine Phosphatase, Non-Receptor Type 6
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Protein Tyrosine Phosphatases
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SH2 Domain-Containing Protein Tyrosine Phosphatases
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Dimethyl Sulfoxide