Abstract
Norepinephrine inhibits omega-conotoxin GVIA-sensitive presynaptic Ca2+ channels in chick dorsal root ganglion neurons through two pathways, one mediated by Go and the other by Gi. These pathways desensitize at different rates. We have found that recombinant Galpha interacting protein (GAIP) and regulators of G protein signaling (RGS)4 selectively accelerate the rate of desensitization of Go- and Gi-mediated pathways, respectively. Blockade of endogenous RGS proteins using antibodies raised against Galpha interacting protein and RGS4 slows the rate of desensitization of these pathways in a selective manner. These results demonstrate that different RGS proteins may interact with Gi and Go selectively, giving rise to distinct time courses of transmitter-mediated effects.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Calcium Channel Blockers / pharmacology
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Calcium Channels / physiology*
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Cells, Cultured
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Chick Embryo
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Electrophysiology
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GTP Phosphohydrolases / physiology
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GTP-Binding Proteins / physiology*
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GTPase-Activating Proteins
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Molecular Sequence Data
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Mollusk Venoms / pharmacology
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Neurons / drug effects
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Neurons / physiology
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Peptides / pharmacology
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Phosphoproteins / physiology
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Presynaptic Terminals / drug effects
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Presynaptic Terminals / physiology*
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Proteins / physiology
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RGS Proteins*
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Signal Transduction*
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omega-Conotoxin GVIA
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ras GTPase-Activating Proteins
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ras Proteins / physiology
Substances
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Calcium Channel Blockers
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Calcium Channels
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GTPase-Activating Proteins
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Mollusk Venoms
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Peptides
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Phosphoproteins
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Proteins
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RGS Proteins
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ras GTPase-Activating Proteins
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regulator of G-protein signalling 19
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RGS4 protein
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omega-Conotoxin GVIA
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GTP Phosphohydrolases
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GTP-Binding Proteins
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ras Proteins
Associated data
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GENBANK/AF090081
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GENBANK/AF090083