39 allergens, chemical ingredients commonly used in cosmetics in China, were tested. The safe concentration of allergens was determined in experiments on animals. On the basis of the above results and referring to the literature, the concentration of allergens used in patch test for human was designed and the patch test was carried out on 10 volunteers by routine method. Finally the concentration which gave no reaction in all 10 volunteers was verified in patients with eczema or contact dermatitis. The rate of positive reactions for each allergen was raged from 0 to 12% with an average 3.4%, According to the results of three above mentioned experiments the series of allergen standards for patch test was established. It is used as an appendix to the document for diagnostic criteria and principles of management of consmetic diseases.