Selective PCR amplification of functional immunoglobulin light chain from hybridoma containing the aberrant MOPC 21-derived V kappa by PNA-mediated PCR clamping

Biotechniques. 1999 May;26(5):818-20, 822. doi: 10.2144/99265bm04.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / genetics
  • Base Sequence
  • Biotechnology
  • DNA Primers / genetics
  • Hybridomas / immunology*
  • Immunoglobulin Variable Region / genetics
  • Immunoglobulin kappa-Chains / genetics*
  • Mice
  • Multiple Myeloma / genetics
  • Multiple Myeloma / immunology
  • Peptide Nucleic Acids / genetics
  • Polymerase Chain Reaction / methods*
  • Rats

Substances

  • Antibodies, Monoclonal
  • DNA Primers
  • Immunoglobulin Variable Region
  • Immunoglobulin kappa-Chains
  • Peptide Nucleic Acids