Abstract
Four stable analogues of methionyl adenylate (3-6) were designed as inhibitors of methionyl-tRNA synthetase and synthesized from 2',3'-isopropylideneadenosine. They strongly inhibited aminoacylation activity of methionyl-tRNA synthetases isolated from Escherichia coli, Mycobacterium tuberculosis, Saccharomyces cerevisiae and human. Among the microorganisms tested, however, these chemicals showed the growth inhibition effect only on E. coli.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Monophosphate / analogs & derivatives*
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Adenosine Monophosphate / chemistry
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Adenosine Monophosphate / pharmacology
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Anti-Bacterial Agents
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Anti-Infective Agents / chemistry
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Anti-Infective Agents / pharmacology*
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Drug Design
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Enzyme Inhibitors / chemistry
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Enzyme Inhibitors / pharmacology*
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Escherichia coli / drug effects
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Escherichia coli / enzymology
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Humans
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Methionine / analogs & derivatives*
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Methionine / chemistry
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Methionine / pharmacology
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Methionine-tRNA Ligase / antagonists & inhibitors*
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Microbial Sensitivity Tests
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Mycobacterium tuberculosis / drug effects
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Mycobacterium tuberculosis / enzymology
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Saccharomyces cerevisiae / drug effects
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Saccharomyces cerevisiae / enzymology
Substances
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Anti-Bacterial Agents
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Anti-Infective Agents
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Enzyme Inhibitors
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methioninyl adenylate
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Adenosine Monophosphate
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Methionine
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Methionine-tRNA Ligase