Abstract
Primers designed in this study were used in a polymerase chain reaction test to amplify a species-specific fragment of approximately 340 bp of the large subunit ribosomal DNA of Debaryomyces hansenii/Candida famata. None of the other medically relevant yeasts including C. guilliermondii, and also the related species, D. nepalensis and C. saitoana, were amplified by this primer pair.
MeSH terms
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Candida / classification*
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Candida / isolation & purification
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DNA, Fungal / analysis
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DNA, Ribosomal / analysis
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Humans
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Mycoses / microbiology
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Polymerase Chain Reaction*
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Saccharomycetales / classification*
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Saccharomycetales / genetics
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Saccharomycetales / isolation & purification
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Species Specificity
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Yeasts / classification
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Yeasts / genetics
Substances
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DNA, Fungal
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DNA, Ribosomal