Colossal crypts bordering colon adenomas in Apc(Min) mice express full-length Apc

Am J Pathol. 1999 Jun;154(6):1831-4. doi: 10.1016/S0002-9440(10)65439-9.

Abstract

Enlarged but nondysplastic crypts are frequently observed at the margins of colon tumors, forming what has been called a transitional epithelium. It is now thought that this is a reactive state and not a preneoplastic condition as previously suggested. We have used the mouse familial adenomatous polyposis model, ApcMin, to study these abnormal adenoma-associated crypts. We report that these nondysplastic crypts are enormous (as much as 10 times normal length) and branch more frequently than normal crypts. They express wild-type Apc protein and display the wild-type Apc allele. We conclude that the colossal crypts at adenoma margins have normal Apc gene function, consistent with the suggestion that their phenotype is a reactive state. The cause remains an open question, but the dramatic epithelial response hints at the presence of potent epithelial trophic factors in the vicinity of colon tumors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoma / genetics
  • Adenoma / metabolism
  • Adenoma / pathology*
  • Adenomatous Polyposis Coli Protein
  • Alleles
  • Animals
  • Cell Differentiation / genetics
  • Colonic Neoplasms / genetics
  • Colonic Neoplasms / metabolism
  • Colonic Neoplasms / pathology
  • Cytoskeletal Proteins / biosynthesis
  • Cytoskeletal Proteins / genetics*
  • Fluorescent Antibody Technique
  • Loss of Heterozygosity
  • Mice
  • Microscopy, Electron, Scanning
  • Polymerase Chain Reaction

Substances

  • Adenomatous Polyposis Coli Protein
  • Cytoskeletal Proteins