Abstract
In this work we eluted peptides from purified class I MHC molecules, isolated from a novel human cervical carcinoma cell line (INBL), generated in our laboratory and positive for HPV-18 infection. A fraction of these peptides was capable of stimulating T lymphocytes obtained from a donor matched for HLA-Cw4 and who was also HPV-18+. Direct N-terminal Edman degradation of these peptides, revealed the sequence (XQFPIFLQF) that matched 85% with the sequence NVFPIFLQM localized in between the 54 and 62 residues of the HPV-18 L1 protein. After stimulation with the synthetic peptide NVFPIFLQM, T lymphocytes from the donor were capable to lyse INBL cells. Our results provide evidence of the existence of naturally occurring viral epitopes presented on cervical cancer cells by the HLA-Cw4 allele, that could be useful for immunotherapy on this type of patient.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Antigens, Viral / chemistry
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Antigens, Viral / immunology
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Cytotoxicity, Immunologic
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Epitopes / immunology
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Female
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Histocompatibility Antigens Class I / chemistry
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Histocompatibility Antigens Class I / immunology*
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Humans
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Lymphocyte Activation
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Mass Spectrometry
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Molecular Sequence Data
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Papillomaviridae / immunology*
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Papillomavirus Infections / immunology
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Papillomavirus Infections / virology
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Peptides / chemistry
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Peptides / immunology*
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Peptides / isolation & purification
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T-Lymphocytes / immunology
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Tumor Cells, Cultured
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Tumor Virus Infections / immunology
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Tumor Virus Infections / virology
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Uterine Cervical Neoplasms / immunology
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Uterine Cervical Neoplasms / virology*
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Viral Proteins / chemistry
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Viral Proteins / immunology*
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Viral Proteins / isolation & purification
Substances
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Antigens, Viral
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Epitopes
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Histocompatibility Antigens Class I
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Peptides
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Viral Proteins