Presenilins interact with Rab11, a small GTPase involved in the regulation of vesicular transport

Hum Mol Genet. 1999 Jul;8(7):1263-9. doi: 10.1093/hmg/8.7.1263.

Abstract

Presenilin 1 (PS1) mutations account for the majority of early-onset dominant cases of familial Alzheimer's disease. Presenilins (PSs) are located in many intra-cellular compartments such as the endoplasmic reticulum, Golgi apparatus, nuclear region and vesicular structures. These proteins include from seven to nine putative transmembrane domains, with the N- and C-terminal ends and a large hydrophilic loop orientated towards the cytoplasm. We report an interaction between the human PS1 or PS2 hydrophilic loop and Rab11, a small GTPase belonging to the Ras-related superfamily. Interaction domains were mapped to codons 374-400 for PS1 and to codons 106-179 for Rab11, a region including the fourth GTP-binding domain. Considering the implication of Rab proteins in vesicular transport pathways, the PS-Rab11 inter-action suggests that PSs might be involved in amyloid precursor protein vesicular routing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Binding Sites
  • Biological Transport / physiology
  • COS Cells
  • GTP Phosphohydrolases / metabolism*
  • GTP-Binding Proteins / metabolism*
  • Humans
  • Membrane Proteins / metabolism*
  • Molecular Sequence Data
  • Presenilin-1
  • Protein Conformation
  • Saccharomyces cerevisiae / metabolism
  • rab GTP-Binding Proteins*

Substances

  • Membrane Proteins
  • PSEN1 protein, human
  • Presenilin-1
  • GTP Phosphohydrolases
  • GTP-Binding Proteins
  • rab11 protein
  • rab GTP-Binding Proteins