Enhancement of (-)-stepholidine on protein phosphorylation of a dopamine- and cAMP-regulated phosphoprotein in denervated striatum of oxidopamine-lesioned rats

Zhongguo Yao Li Xue Bao. 1998 Mar;19(2):100-3.

Abstract

Aim: To study effects of (-)-stepholidine (SPD) on the phosphorylation of a dopamine- and cAMP-regulated phosphoprotein (DARPP-32) in the striatum of oxidopamine-lesioned rats.

Methods: The amount of dephospho-DARPP-32 was measured by a back-phosphorylation assay.

Results: In the striatum of control rats, SPD per se had no effect on the phosphorylation of DARPP-32, but it antagonized the decrease by 28% of dephospho-DARPP-32 induced by the D1 agonist SK&F-38393. In the denervated striatum of oxidopamine-lesioned rats, SPD decreased the amount of dephospho-DARPP-32 by 44%. The effect of SPD was completely counteracted by the concomitant administration of the D1 antagonist Sch-23390.

Conclusion: SPD exhibits D1 agonistic action on DARPP-32 phosphorylation in the denervated striatum of oxidopamine-lesioned rats, but it acts as a D1 antagonist in normal striatum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine / antagonists & inhibitors
  • Animals
  • Benzazepines / pharmacology
  • Berberine / analogs & derivatives*
  • Berberine / pharmacology
  • Corpus Striatum / metabolism*
  • Dopamine Agonists
  • Dopamine Antagonists / pharmacology*
  • Dopamine and cAMP-Regulated Phosphoprotein 32
  • Enzyme Inhibitors / metabolism
  • Male
  • Nerve Tissue Proteins / metabolism*
  • Oxidopamine
  • Phosphoproteins / metabolism*
  • Phosphorylation / drug effects
  • Rats
  • Rats, Sprague-Dawley

Substances

  • Benzazepines
  • Dopamine Agonists
  • Dopamine Antagonists
  • Dopamine and cAMP-Regulated Phosphoprotein 32
  • Enzyme Inhibitors
  • Nerve Tissue Proteins
  • Phosphoproteins
  • Berberine
  • stepholidine
  • 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine
  • Oxidopamine