Aim: To examine the possible inhibitory role of ciclosporin (Cic) on luminol-dependent chemiluminescence (CL) of whole blood in renal transplant patients.
Methods: Luminol-dependent CL was used to measure active oxygen species generation in respiratory burst of whole blood stimulated by zymosan A. Fluorescence polarization immunoassay was used to monitor the blood concentration of Cic.
Results: CL values of Cic group (n = 50) decreased in comparison with those of normal group (n = 10) (P < 0.01). The blood concentration of Cic was negatively related to CL value (P < 0.01). The serum of renal transplant patients directly inhibited respiratory burst of peritoneal macrophages of rats in a concentration-dependent manner.
Conclusion: Cic inhibits the phagocytic activity of neutrophils in renal transplant patients.