Abstract
This study was performed to investigate the effects of isoprothiolane on cell growth and the production of interleukin (IL)-1 and IL-6 by bovine mammary epithelial cells in vitro. Isoprothiolane increased proliferation of mammary epithelial cells in a dose-dependent manner at the concentration of 0.05 to 5 microM when cultured either with or without serum-supplemented medium. In contrast, isoprothiolane (0.0005-5 microM) significantly inhibited the production of IL-1 and IL-6 by mammary epithelial cells. Moreover, the cytokines, IL-1alpha, IL-1beta, IL-6, and tumor necrosis factor (TNF)-alpha tended to inhibit the proliferation of mammary epithelial cells in a dose-dependent manner. These results indicated that isoprothiolane regulated mammary epithelial cell growth in vitro possibly by modulating the production of cytokines.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
Cattle
-
Cell Division / drug effects
-
Cells, Cultured
-
Epithelial Cells / cytology*
-
Epithelial Cells / drug effects
-
Epithelial Cells / physiology
-
Female
-
Fungicides, Industrial / pharmacology*
-
Gene Expression Regulation / drug effects
-
Glyceraldehyde-3-Phosphate Dehydrogenases / genetics
-
Humans
-
Interleukin-1 / genetics
-
Interleukin-1 / pharmacology
-
Interleukin-6 / genetics
-
Interleukin-6 / pharmacology
-
Mammary Glands, Animal / cytology*
-
Mammary Glands, Animal / physiology
-
Recombinant Proteins / pharmacology
-
Reverse Transcriptase Polymerase Chain Reaction
-
Thiophenes / pharmacology*
-
Transcription, Genetic / drug effects
-
Tumor Necrosis Factor-alpha / pharmacology
Substances
-
Fungicides, Industrial
-
Interleukin-1
-
Interleukin-6
-
Recombinant Proteins
-
Thiophenes
-
Tumor Necrosis Factor-alpha
-
isoprothiolane
-
Glyceraldehyde-3-Phosphate Dehydrogenases