Abstract
Complementation experiments, Tn5 mutagenesis, and DNA sequencing were used to identify a locus (lag-1) that participates in acetylation of Legionella pneumophila serogroup 1 lipopolysaccharide. Nuclear magnetic resonance analyses of lipopolysaccharides from mutant and complemented strains suggest that lag-1 is responsible for O acetylation of serogroup 1 O polysaccharide.
MeSH terms
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Acetylation
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Acetyltransferases
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Bacterial Proteins
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Carbohydrate Sequence
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Cloning, Molecular
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Genes, Bacterial*
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Genetic Complementation Test
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Legionella pneumophila / classification
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Legionella pneumophila / genetics*
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Molecular Sequence Data
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Mutation
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Nuclear Magnetic Resonance, Biomolecular
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O Antigens / biosynthesis*
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O Antigens / chemistry
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Peptides / genetics*
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Polysaccharides / metabolism
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Sequence Analysis
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Serotyping
Substances
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Bacterial Proteins
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O Antigens
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Peptides
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Polysaccharides
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lag-1 protein, Legionella
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Acetyltransferases