Abstract
Key regulatory regions necessary for the expression of the gene encoding FcepsilonRI alpha-chain, a component of the high-affinity IgE receptor primarily responsible for IgE-dependent allergic response, were investigated. Two regions, -74/-69 and -55/-47, which contained binding motifs for proteins belonging to the Ets family and the GATA family, respectively, were shown to be necessary for the activation of the alpha-chain promoter. Both the regulatory elements enhanced the promoter activity only in alpha-chain-producing cells PT18 and RBL-2H3 (mast cell lines), indicating that the elements required specific trans-acting proteins present in the alpha-chain-producing cells. EMSA using nuclear extracts and in vitro-translated proteins revealed that Elf-1 and GATA-1 bound to the enhancer elements. This is the first report describing the regulation in the expression of the FcepsilonRI alpha-chain.
MeSH terms
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Animals
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Cell Line
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Cell Nucleus / genetics
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Cell Nucleus / metabolism
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Enhancer Elements, Genetic / immunology*
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Ephrin-A2
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Erythroid-Specific DNA-Binding Factors
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GATA1 Transcription Factor
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Humans
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Jurkat Cells
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Mast Cells / metabolism
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Mice
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Mutagenesis, Site-Directed
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Promoter Regions, Genetic / immunology
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Protein Binding / immunology
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Proto-Oncogene Proteins / genetics
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Proto-Oncogene Proteins / metabolism
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Proto-Oncogene Proteins c-ets
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Rats
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Receptors, IgE / biosynthesis
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Receptors, IgE / genetics*
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Receptors, IgE / metabolism*
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcriptional Activation / immunology
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Up-Regulation / genetics
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Up-Regulation / immunology
Substances
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DNA-Binding Proteins
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Ephrin-A2
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Erythroid-Specific DNA-Binding Factors
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GATA1 Transcription Factor
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GATA1 protein, human
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Gata1 protein, mouse
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Gata1 protein, rat
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Proto-Oncogene Proteins
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Proto-Oncogene Proteins c-ets
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Receptors, IgE
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Transcription Factors