Abstract
During telophase, Golgi cisternae are regenerated and stacked from a heterogeneous population of tubulovesicular clusters. A cell-free system that reconstructs these events has revealed that cisternal regrowth requires interplay between soluble factors and soluble N-ethylmaleimide (NEM)-sensitive fusion protein (NSF) attachment protein receptors (SNAREs) via two intersecting pathways controlled by the ATPases, p97 and NSF. Golgi reassembly stacking protein 65 (GRASP65), an NEM-sensitive membrane-bound component, is required for the stacking process. NSF-mediated cisternal regrowth requires a vesicle tethering protein, p115, which we now show operates through its two Golgi receptors, GM130 and giantin. p97-mediated cisternal regrowth is p115-independent, but we now demonstrate a role for p115, in conjunction with its receptors, in stacking p97 generated cisternae. Temporal analysis suggests that p115 plays a transient role in stacking that may be upstream of GRASP65-mediated stacking. These results implicate p115 and its receptors in the initial alignment and docking of single cisternae that may be an important prerequisite for stack formation.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenosine Triphosphatases
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Animals
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Autoantigens
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Carrier Proteins / analysis
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Carrier Proteins / physiology*
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Cell Cycle Proteins / analysis
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Cell Cycle Proteins / physiology
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Centrifugation, Density Gradient
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Cytosol / chemistry
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Cytosol / drug effects
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Cytosol / metabolism
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Ethylmaleimide / pharmacology
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Golgi Apparatus / drug effects
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Golgi Apparatus / metabolism*
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Golgi Apparatus / ultrastructure
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Golgi Matrix Proteins
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HeLa Cells
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Humans
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Intracellular Membranes / drug effects
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Intracellular Membranes / metabolism*
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Intracellular Membranes / ultrastructure
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Kinetics
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Liver / cytology
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Liver / metabolism
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Membrane Fusion / drug effects
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Membrane Proteins / analysis
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Membrane Proteins / physiology*
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Mitosis*
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N-Ethylmaleimide-Sensitive Proteins
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Peptide Fragments / physiology
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Rats
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SNARE Proteins
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Solubility
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Valosin Containing Protein
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Vesicular Transport Proteins*
Substances
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Autoantigens
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Carrier Proteins
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Cell Cycle Proteins
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GORASP1 protein, human
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Golgi Matrix Proteins
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Golgin subfamily A member 2
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Gorasp1 protein, rat
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Membrane Proteins
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Peptide Fragments
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SNARE Proteins
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Vesicular Transport Proteins
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macrogolgin
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vesicular transport factor p115
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Adenosine Triphosphatases
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N-Ethylmaleimide-Sensitive Proteins
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Nsf protein, rat
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Valosin Containing Protein
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Ethylmaleimide