Abstract
The amphicrine AR42J acinar cell line is an excellent model to study both exocrine and neuroendocrine exocytotic mechanisms. As a first step toward this goal, we determined the specific isoforms of the v- and t-SNARE and Munc18 families expressed in these cells. In addition, we show that dexamethasone-induced differentiation toward the exocrine phenotype causes an upregulation of several of these proteins. AR42J is notoriously difficult to transfect, limiting its usefulness as a model. However, we have now overcome this obstacle by acheiving high efficiency expression of a beta-galactosidase reporter gene and truncated SNAP-25 gene using adenoviral infection techniques. The AR42J cells can now be used to pursue and elucidate the distinct functions of individual SNARE isoforms used in endocrine and exocrine secretion within a single cell line.
Copyright 1999 Academic Press.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adenoviridae / genetics
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Animals
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Brain / cytology
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Brain / metabolism
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Cell Differentiation / drug effects
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Cell Line
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Cell Membrane / metabolism
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Dexamethasone / pharmacology
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Gene Expression* / drug effects
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Genes, Reporter / genetics
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Intracellular Membranes / metabolism
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Male
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Membrane Proteins / genetics
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Membrane Proteins / metabolism*
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Munc18 Proteins
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Nerve Tissue Proteins / genetics
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Nerve Tissue Proteins / metabolism
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Pancreas / cytology*
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Pancreas / drug effects
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Pancreas / metabolism
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Protein Isoforms / genetics
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Protein Isoforms / metabolism
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Qa-SNARE Proteins
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R-SNARE Proteins
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Rats
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Rats, Sprague-Dawley
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SNARE Proteins
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Sequence Deletion
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Synaptosomal-Associated Protein 25
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Transfection*
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Vesicular Transport Proteins*
Substances
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Membrane Proteins
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Munc18 Proteins
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Nerve Tissue Proteins
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Protein Isoforms
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Qa-SNARE Proteins
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R-SNARE Proteins
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SNARE Proteins
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Snap25 protein, rat
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Synaptosomal-Associated Protein 25
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Vesicular Transport Proteins
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Dexamethasone